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Properties
of TiO2-supported bilayers
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The
work
described in this page was performed in collaboration
with the the BioInterface
Group
at the Swiss Federal Institute of Technology (Zürich) within the
context of the Doctoral thesis of Fernanda F. Rossetti Interactions
of Lipidic Assemblies with
Metal Oxide Surfaces and Brush-like Polyelectrolytes.
This page was jointly
designed by F. F. Rossetti and I. Reviakine.
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Asymmetric
distribution of phosphatidyl serine in TiO2-supported
bilayers.
In this scheme, a liposome composed
of phosphatidyl serine (PS, green) and phosphatidyl choline (red) is
shown to adsorb on the TiO2 surface in the presence of Ca2+.
The bilayers that form are asymmetric with respect to the distribution
of PS between the two bilayer leaflets: most of PS is sequestered in
the surface-facing (proximal) leaflet and its mobility is restricted.
Fluorescence recovery after photobleaching (FRAP) was used to
investigate lipid mobility. In a typical FRAP experiment, an area of a
bilayer is bleached with a high-intensity laser beam, and the recovery
of fluorescence intensity in this area is followed. The bleached area
is shown in gray. Due to the restricted mobility of PS in the proximal
leaflet of these bilayers, the recovery of the fluorescence intensity
is slowed down tremendously when fluorescently labeled PS is used as a
label.
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| The figure above was designed by Dr.
Fernanda Rossetti.
Details of the work can be found in Rossetti et al., Langmuir 2006, 22, 3467. 10.1021/la053000r |
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If you are interested in the subject of
lipid asymmetry in supported lipid bilayers, you may wish to look at
the following publications:
- Richter et al., "Formation of Solid-Supported Lipid
Bilayers: An Integrated View" Langmuir 2006, 22, 3497. 10.1021/la052687c
- Richter et al. "On the Effect of the Solid Support on the
Interleaflet Distribution of Lipids in Supported Lipid Bilayers."
Langmuir 2005,
21:
299-304. 10.1021/la0478402
- Crane et al. "Measuring lipid asymmetry in planar
supported bilayers by fluorescence interference contrast microscopy." Langmuir 2005, 21:1377-1388. 10.1021/la047654w
- Kasbauer et al. "Effect of cationic lipids
in the formation of asymmetries in supported bilayers.", Biophys. J. 1999, 76:2600-2605. Link
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