Sonicated EggPC vesicles adsorbed on
the surface of mica in EDTA buffer. 0.5 mg/ml lipid The vesicles are
seen to remain intact.
Contact mode image, 1.5 x 1.5 µm.
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Extruded, r ~ 25 nm EggPC vesicles
adsorbed on the surface of mica in EDTA buffer. 3 mg/ml lipid. In the
insets, images of several and of one vesicle are shown. The sample was
washed with a solution of a protein, Cholera Toxin Subunit B5
(take a look here),
to fix the vesicles on the
surface (the idea is due to Shao et al). The protein molecules
can be seen
surrounding the vesicle in the right inset. Contact mode images, 5 x 5 µm,
right inset: x100x110 nm.
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Extruded, r ~ 50 nm EggPC vesicles
adsorbed on the surface of mica in EDTA buffer. Two kinds of objects
are visible: single bilayer disks and intact vesicles (green and
turquoise arrowheads, respectively). 0.006 mg/ml lipid. Contact mode
image, 3 x 3 µm.
Size analysis of
both kinds of objects revealed that vesicles with r > 75 nm
ruptured, while those with r < 75 nm remained intact. See Reviakine
and Brisson, Langmuir
17 8293 (2002),
for detailed discussion and comparison with theoretical work.
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